Lesson 3: DNA Replication - Definitions

The current accepted model for replication is semi-conservative replication. This involves replicating all DNA before mitosis or meiosis occurs

Definitions

Complementary base pairing is where the bonding of the bases on one strand corresponds with the bases on the opposite strand.

DNA polymerase forms covalent bonds between the nucleotides on the newly forming DNA, a phosphodiester bond is formed in a condensation reaction. It only works in the [3′ –> 5′] direction.

The leading strand is a strand on DNA that runs [3′ –> 5′], DNA polymerase attaches to the strand and follows the helicase is one go.

The lagging strand runs [5′ –> 3′] – therefore DNA polymerase runs along it in sections.

Okazki fragments are the sections of DNA formed when polymerase completes a section (working backwards) and then it falls off. Another DNA polymerase joins to the strand and it continues.

The template strand is the strand of DNA which the nucleotides are attached to

RNA primase primes the DNA strands so they can join together

DNA ligase joins the two strands of DNA together